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KMID : 0861020100250020099
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Korea Journal of Herbology
2010 Volume.25 No. 2 p.99 ~ p.106
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Anti-oxidative effects of fractionated Pulsatilla koreana NAKAI extracts
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Cho Hyun-Jin
Yun Hyun-Jeong
Yi Hyo-Seung
Park Sun-Dong
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Abstract
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Objectives : This study was designed to investigate the effects of anti-oxidation of fractionated Pulsatilla koreana NAKAI (PK) extracts. And we examined to determine that a certain fractionated extract has the best anti-oxidative effects between the fractionated PK extracts.
Methods : Anti-oxidative effects of fractionated PK extracts was measured by scavenging activities of DPPH, superoxide, nitric oxide (NO) and peroxynitrite radicals. And also scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cell was measured. After these examination, we determined a fraction that has best anti-oxidative effects.
Results : Fractionated PK extracts inhibited radicals effectively. Also in RAW 264.7 cell, intracellular oxidation has inhibited by PK extracts. In these tests, ethyl acetate (EA) fraction has the best anti-oxidative effects among PK extracts.
Conclusions : This results demonstrate that PK extracts exhibit anti-oxidative effects. And EA fraction has the best inhibition effects among the six fractions of PK.
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KEYWORD
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Pulsatilla koreana NAKAI, anti-oxidation, DPPH, superoxide, nitric oxide, Peroxynitrite
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